Study of porcine hepatocyte-entrapped bioartificial liver in surgery-induced fulminant hepatic failure rabbits

Chi Chang Lin; Chih Chi Wang; Kuo Chen Hung; Chao Long Chen; Chee Chien Yong; Tai Horng Young; Eiji Kobayash

doi:10.1016/j.bej.2013.04.006

Study of porcine hepatocyte-entrapped bioartificial liver in surgery-induced fulminant hepatic failure rabbits

Chi Chang Lin, Chih Chi Wang^*, Kuo Chen Hung, Chao Long Chen, Chee Chien Yong, Tai Horng Young, Eiji Kobayash

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2 引文斯高帕斯（Scopus）

摘要

ICP is an important cause of mortality in fulminant hepatic failure. The aim of this study was to prolonged survival in surgery-induced FHF rabbit with BAL. Four hours after induction of FHF, rabbits were connected to a 4-h whole blood perfusion through the BAL containing 1.2×10⁹ fresh isolated porcine hepatocytes (estimated 30% of liver volume of rabbit) via an arterial-venous shunt. Survival times in control group, sham-BAL group and BAL group were14.4±4.4h, 12.6±2.6h and 22.6±5.2h, respectively (BAL vs. control, p=0.026; BAL vs. sham-BAL, p=0.006). BAL group remain ICP level less than 10mmHg for near 13h after FHF induction while 19 and 15mmHg were observed in control and sham-BAL groups, respectively. BAL containing porcine hepatocytes significantly prolonged survival time by delayed increase of intracranial pressure compared with the control and sham-BAL groups.

原文	英語
頁（從 - 到）	55-59
頁數	5
期刊	Biochemical Engineering Journal
卷	76
DOIs	https://doi.org/10.1016/j.bej.2013.04.006
出版狀態	已出版 - 05 07 2013

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10.1016/j.bej.2013.04.006

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@article{2e981adf979c41baa993886accb77405,

title = "Study of porcine hepatocyte-entrapped bioartificial liver in surgery-induced fulminant hepatic failure rabbits",

abstract = "ICP is an important cause of mortality in fulminant hepatic failure. The aim of this study was to prolonged survival in surgery-induced FHF rabbit with BAL. Four hours after induction of FHF, rabbits were connected to a 4-h whole blood perfusion through the BAL containing 1.2×109 fresh isolated porcine hepatocytes (estimated 30% of liver volume of rabbit) via an arterial-venous shunt. Survival times in control group, sham-BAL group and BAL group were14.4±4.4h, 12.6±2.6h and 22.6±5.2h, respectively (BAL vs. control, p=0.026; BAL vs. sham-BAL, p=0.006). BAL group remain ICP level less than 10mmHg for near 13h after FHF induction while 19 and 15mmHg were observed in control and sham-BAL groups, respectively. BAL containing porcine hepatocytes significantly prolonged survival time by delayed increase of intracranial pressure compared with the control and sham-BAL groups.",

keywords = "Animal model, Bioartificial liver, Fulminant hepatic failure, Hepatocyte, Hollow fiber, Intracranial pressure",

author = "Lin, {Chi Chang} and Wang, {Chih Chi} and Hung, {Kuo Chen} and Chen, {Chao Long} and Yong, {Chee Chien} and Young, {Tai Horng} and Eiji Kobayash",

year = "2013",

month = jul,

day = "5",

doi = "10.1016/j.bej.2013.04.006",

language = "英语",

volume = "76",

pages = "55--59",

journal = "Biochemical Engineering Journal",

issn = "1369-703X",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Study of porcine hepatocyte-entrapped bioartificial liver in surgery-induced fulminant hepatic failure rabbits

AU - Lin, Chi Chang

AU - Wang, Chih Chi

AU - Hung, Kuo Chen

AU - Chen, Chao Long

AU - Yong, Chee Chien

AU - Young, Tai Horng

AU - Kobayash, Eiji

PY - 2013/7/5

Y1 - 2013/7/5

N2 - ICP is an important cause of mortality in fulminant hepatic failure. The aim of this study was to prolonged survival in surgery-induced FHF rabbit with BAL. Four hours after induction of FHF, rabbits were connected to a 4-h whole blood perfusion through the BAL containing 1.2×109 fresh isolated porcine hepatocytes (estimated 30% of liver volume of rabbit) via an arterial-venous shunt. Survival times in control group, sham-BAL group and BAL group were14.4±4.4h, 12.6±2.6h and 22.6±5.2h, respectively (BAL vs. control, p=0.026; BAL vs. sham-BAL, p=0.006). BAL group remain ICP level less than 10mmHg for near 13h after FHF induction while 19 and 15mmHg were observed in control and sham-BAL groups, respectively. BAL containing porcine hepatocytes significantly prolonged survival time by delayed increase of intracranial pressure compared with the control and sham-BAL groups.

AB - ICP is an important cause of mortality in fulminant hepatic failure. The aim of this study was to prolonged survival in surgery-induced FHF rabbit with BAL. Four hours after induction of FHF, rabbits were connected to a 4-h whole blood perfusion through the BAL containing 1.2×109 fresh isolated porcine hepatocytes (estimated 30% of liver volume of rabbit) via an arterial-venous shunt. Survival times in control group, sham-BAL group and BAL group were14.4±4.4h, 12.6±2.6h and 22.6±5.2h, respectively (BAL vs. control, p=0.026; BAL vs. sham-BAL, p=0.006). BAL group remain ICP level less than 10mmHg for near 13h after FHF induction while 19 and 15mmHg were observed in control and sham-BAL groups, respectively. BAL containing porcine hepatocytes significantly prolonged survival time by delayed increase of intracranial pressure compared with the control and sham-BAL groups.

KW - Animal model

KW - Bioartificial liver

KW - Fulminant hepatic failure

KW - Hepatocyte

KW - Hollow fiber

KW - Intracranial pressure

UR - http://www.scopus.com/inward/record.url?scp=84877353673&partnerID=8YFLogxK

U2 - 10.1016/j.bej.2013.04.006

DO - 10.1016/j.bej.2013.04.006

M3 - 文章

AN - SCOPUS:84877353673

SN - 1369-703X

VL - 76

SP - 55

EP - 59

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

ER -

Study of porcine hepatocyte-entrapped bioartificial liver in surgery-induced fulminant hepatic failure rabbits

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