Surfactant effects on lipase-catalyzed hydrolysis of olive oil in AOT/ISOOCTANE reverse micelles

Shau Wei Tsai*, Kung Ping Lee, Chen Li Chiang

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

The effects of surfactant concentration on the hydrolytic activity of Candida rugosa lipase in AOT/isooctane reverse micelles with olive oil as the substrate has been investigated. A noncompetitive inhibition by the surfactant on the enzyme was observed. Strong dependences of the kinetic constants kcat and kM, but not kI on the water-to-surfactant ratio (R value) have been identified. The benefits of carrying out the hydrolysis at higher surfactant and water concentrations were demonstrated from the improvement of the initial rate and time course of conversion.

原文英語
頁(從 - 到)89-98
頁數10
期刊Biocatalysis and Biotransformation
13
發行號2
DOIs
出版狀態已出版 - 1995
對外發佈

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