TY - JOUR
T1 - Synergic effect of combined xenogeneic mesenchymal stem cells and ceftriaxone on acute septic arthritis
AU - Sung, Pei Hsun
AU - Yin, Tsung Cheng
AU - Chiang, John Y.
AU - Chen, Chih Hung
AU - Huang, Chi Ruei
AU - Lee, Mel S.
AU - Yip, Hon Kan
N1 - © The Author(s) 2024. Published by Oxford University Press.
PY - 2024/8/16
Y1 - 2024/8/16
N2 - BACKGROUND: This study tested the hypothesis that combined ceftriaxone (Cef) and human umbilical cord-derived mesenchymal stem cells (HUCDMSCs) was better than either therapy for alleviating acute septic arthritis (ASA).METHODS AND RESULTS: Adult-male C57BL/6 mice were categorized into control group (Clt), group A (ASA only), group B [ASA + Cef (5 mg/kg, IM per day, at days 2 to 16 after ASA induction)], group C [ASA + HUCDMSCs (5 × 105 per mice at days 2, 3, 4 after ASA induction)], and group D (ASA + Cef + HUCDMSCs). Animals were euthanized by day 28. The result demonstrated that the body weight was significantly lower, whereas the ratio of kidney or spleen weight to WB, circulatory WBC count, bacterial colony-formation-unit from circulatory/kidney extraction were significantly higher in group A than in other groups (all P < .001). The proinflammatory cytokines (IL-6/TNF-α) of knee joint fluid were lowest in Clt and significantly and progressively reduced from groups A to D, whereas the circulatory levels of these 2 parameters at the time points of days 3/7/28 exhibited an identical pattern as knee joint fluid among the groups (all P-value < .0001). The scores of vertebral-bone destructions/inflamed synovium were lowest in Clt, highest in group A, significantly higher in group C than in groups B/D, and significantly higher in group C than in group D (all P < .0001).CONCLUSION: Combined antibiotics and Cef and HUCDMSCs was superior to just one therapy for suppressing circulatory and tissue levels of inflammation and knee joint destruction in ASA.
AB - BACKGROUND: This study tested the hypothesis that combined ceftriaxone (Cef) and human umbilical cord-derived mesenchymal stem cells (HUCDMSCs) was better than either therapy for alleviating acute septic arthritis (ASA).METHODS AND RESULTS: Adult-male C57BL/6 mice were categorized into control group (Clt), group A (ASA only), group B [ASA + Cef (5 mg/kg, IM per day, at days 2 to 16 after ASA induction)], group C [ASA + HUCDMSCs (5 × 105 per mice at days 2, 3, 4 after ASA induction)], and group D (ASA + Cef + HUCDMSCs). Animals were euthanized by day 28. The result demonstrated that the body weight was significantly lower, whereas the ratio of kidney or spleen weight to WB, circulatory WBC count, bacterial colony-formation-unit from circulatory/kidney extraction were significantly higher in group A than in other groups (all P < .001). The proinflammatory cytokines (IL-6/TNF-α) of knee joint fluid were lowest in Clt and significantly and progressively reduced from groups A to D, whereas the circulatory levels of these 2 parameters at the time points of days 3/7/28 exhibited an identical pattern as knee joint fluid among the groups (all P-value < .0001). The scores of vertebral-bone destructions/inflamed synovium were lowest in Clt, highest in group A, significantly higher in group C than in groups B/D, and significantly higher in group C than in group D (all P < .0001).CONCLUSION: Combined antibiotics and Cef and HUCDMSCs was superior to just one therapy for suppressing circulatory and tissue levels of inflammation and knee joint destruction in ASA.
KW - acute sepsis arthritis
KW - inflammation
KW - knee joint
KW - vertebral destruction
KW - Humans
KW - Mice, Inbred C57BL
KW - Male
KW - Arthritis, Infectious/drug therapy
KW - Mesenchymal Stem Cells/metabolism
KW - Anti-Bacterial Agents/pharmacology
KW - Mesenchymal Stem Cell Transplantation/methods
KW - Ceftriaxone/pharmacology
KW - Animals
KW - Mice
UR - http://www.scopus.com/inward/record.url?scp=85201725548&partnerID=8YFLogxK
U2 - 10.1093/stcltm/szae034
DO - 10.1093/stcltm/szae034
M3 - 文章
C2 - 38894649
AN - SCOPUS:85201725548
SN - 2157-6564
VL - 13
SP - 724
EP - 737
JO - Stem Cells Translational Medicine
JF - Stem Cells Translational Medicine
IS - 8
ER -