The correlation between TWIST, E-cadherin, and beta-catenin in human bladder cancer

Cheng Huang Shen, Jiann Der Wu, Yeong Chin Jou, Ming Chin Cheng, Chang Te Lin, Pi Che Chen, Ya Shih Tseng, Chung Sheng Shi, Syue Yi Chen, De Ching Chang, Ying Ray Lee*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

22 引文 斯高帕斯(Scopus)

摘要

Purpose: Epithelial-to-mesenchymal transition (EMT)-related factors are known to contribute to the invasion and migration of multiple cancers. However, the expression levels of and the relationship between TWIST, E-cadherin, and beta-catenin in bladder cancer are not yet known. Therefore, this study investigated the relationship between TWIST, E-cadherin, and beta-catenin in tissue specimens and cell lines of bladder cancer. Methods: Microarrays of bladder cancer tissue and bladder cancer cell lines were used to study the expression levels of TWIST, E-cadherin, and beta-catenin, with disease stage and grade using immunohistochemistry. Moreover, the siRNAs of TWIST, E-cadherin, and beta-catenin were transfected into the bladder cancer cell lines to study any relationship between these factors. Results: The levels of TWIST and beta-catenin were up-regulated with increasing grade of malignancy. In contrast, the corresponding results for E-cadherin were just the opposite. Furthermore, inhibition of the expression of TWIST elevated the expression of E-cadherin, but reduced the expression of beta-catenin. However, reduction ofbeta-catenin by siRNA had no influence on TWIST, but up-regulated the expression of E-cadherin. Conclusion: TWIST may act upstream of E-cadherin, which can indirectly regulate the expression levels of beta-catenin. The EMT factors TWIST, E-cadherin, and beta-catenin may be a cluster of biomarkers for the metastatic progression of bladder cancer.

原文英語
頁(從 - 到)733-737
頁數5
期刊Journal of B.U.ON.
16
發行號4
出版狀態已出版 - 10 2011

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