The detection of Gardnerella vaginalis DNA sequences in uncultured clinical specimens with cloned G. vaginalis DNA as probes

Chia C. Pao; Shyh Shyan Lin; T'sang T.ang Hsieh

doi:10.1016/0890-8508(90)90027-W

The detection of Gardnerella vaginalis DNA sequences in uncultured clinical specimens with cloned G. vaginalis DNA as probes

Chia C. Pao^*, Shyh Shyan Lin, T'sang T.ang Hsieh

^*此作品的通信作者

Chang Gung University

研究成果: 期刊稿件 › 文章 › 同行評審

3 引文斯高帕斯（Scopus）

摘要

Cloned Gardnerella vaginalis DNA were selected from a plasmid DNA library constructed with partially restriction endonuclease Hind III-digested genomic DNA of G. vaginalis to serve as DNA probe in detecting G. vaginalis. The level of detection was determined to be approximately 10,000 cells by slot-blot DNA hybridization. This probe DNA will not cross-hybridize with DNA of a number of non-Gardnerella micro-organisms commonly found in female genital tract. The DNA probe-based hybridization test may become a useful tool for the identification of G. vaginalis in uncultured clinical specimens.

原文	英語
頁（從 - 到）	367-373
頁數	7
期刊	Molecular and Cellular Probes
卷	4
發行號	5
DOIs	https://doi.org/10.1016/0890-8508(90)90027-W
出版狀態	已出版 - 10 1990

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10.1016/0890-8508(90)90027-W

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title = "The detection of Gardnerella vaginalis DNA sequences in uncultured clinical specimens with cloned G. vaginalis DNA as probes",

abstract = "Cloned Gardnerella vaginalis DNA were selected from a plasmid DNA library constructed with partially restriction endonuclease Hind III-digested genomic DNA of G. vaginalis to serve as DNA probe in detecting G. vaginalis. The level of detection was determined to be approximately 10,000 cells by slot-blot DNA hybridization. This probe DNA will not cross-hybridize with DNA of a number of non-Gardnerella micro-organisms commonly found in female genital tract. The DNA probe-based hybridization test may become a useful tool for the identification of G. vaginalis in uncultured clinical specimens.",

keywords = "DNA probes, Gardnerella vaginalis, bacterial vaginosis",

author = "Pao, {Chia C.} and Lin, {Shyh Shyan} and Hsieh, {T'sang T.ang}",

year = "1990",

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doi = "10.1016/0890-8508(90)90027-W",

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AU - Pao, Chia C.

AU - Lin, Shyh Shyan

AU - Hsieh, T'sang T.ang

PY - 1990/10

Y1 - 1990/10

N2 - Cloned Gardnerella vaginalis DNA were selected from a plasmid DNA library constructed with partially restriction endonuclease Hind III-digested genomic DNA of G. vaginalis to serve as DNA probe in detecting G. vaginalis. The level of detection was determined to be approximately 10,000 cells by slot-blot DNA hybridization. This probe DNA will not cross-hybridize with DNA of a number of non-Gardnerella micro-organisms commonly found in female genital tract. The DNA probe-based hybridization test may become a useful tool for the identification of G. vaginalis in uncultured clinical specimens.

AB - Cloned Gardnerella vaginalis DNA were selected from a plasmid DNA library constructed with partially restriction endonuclease Hind III-digested genomic DNA of G. vaginalis to serve as DNA probe in detecting G. vaginalis. The level of detection was determined to be approximately 10,000 cells by slot-blot DNA hybridization. This probe DNA will not cross-hybridize with DNA of a number of non-Gardnerella micro-organisms commonly found in female genital tract. The DNA probe-based hybridization test may become a useful tool for the identification of G. vaginalis in uncultured clinical specimens.

KW - DNA probes

KW - Gardnerella vaginalis

KW - bacterial vaginosis

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U2 - 10.1016/0890-8508(90)90027-W

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AN - SCOPUS:0025506533

SN - 0890-8508

VL - 4

SP - 367

EP - 373

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 5

ER -

The detection of Gardnerella vaginalis DNA sequences in uncultured clinical specimens with cloned G. vaginalis DNA as probes

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