The growth of mouse osteoblast cell under vibration wave in vitro

Osteoporosis is gradually becoming the number one invisible killer in this current aging society. Along with the extension of human life-span, osteoporosis turned into the second major epidemic disease which is lower to cardiovascular diseases. Chemical medicine is the usual prescription, but its side effect should not be overlooked. Therefore, some researchers discuss the treatment of osteoporosis by using physical stimulation. Low capacity supersonic stimulation to the bone cell has been widely studied and it has been in the stage of clinical application. But few report discussed about the best range of energy impulses to the bone cell. According to the literature, when supersonic energy act on the organism, the propagation of the sound wave will create heat, vibration, and massage effect to the tissue and will provide both constructive and destructive physical therapy affects. Osteoblast cell is a mononuclear cell which can cooperate with other cells to generate or rebuild osteoid of the skeleton. This research studies the culture of mice MC3T3 osteoblast cell in vitro, which stimulates the growing cell with mechanical broad range of frequency (20 KHz to 10 MHz) without temperature factor and investigates the effect of different frequency, amplitude, excitation duration, and wave form of the stimulation. The results show that several different parameters of the vibration excitation have the positive effect onto the osteoblast cell proliferation, and the density of the bone is also increased remarkably.