The healing of critical-sized femoral segmental bone defects in rabbits using baculovirus-engineered mesenchymal stem cells

Chin Yu Lin, Yu Han Chang, Kun Ju Lin, Tzu Chen Yen, Ching Lung Tai, Chi Yuan Chen, Wen Hsin Lo, Ing Tsung Hsiao, Yu Chen Hu*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

78 引文 斯高帕斯(Scopus)

摘要

Management of massive segmental bone defects remains a challenging clinical problem and bone marrow-derived mesenchymal stem cells (BMSCs) hold promise for bone regeneration. To explore whether BMSCs engineered by baculovirus (an emerging gene delivery vector) can heal large bone defects, New Zealand White (NZW) rabbit BMSCs were transduced with the BMP2-expressing baculovirus or VEGF-expressing baculovirus, and co-implanted into critical-sized (10 mm) femoral segmental defects in NZW rabbits. X-ray analysis revealed that the baculovirus-engineered BMSCs not only bridged the defects at as early as week 2, but also healed the defects in 100% of rabbits (13/13) at week 4. The osteogenic metabolism, as monitored by positron emission tomography (PET) also suggested the completion of bone healing at week 8. When compared with other control groups, the BMP2/VEGF-expressing BMSCs remarkably enhanced the segmental bone repair and mechanical properties, as evidenced by micro-computed tomography (μCT), histochemical staining and biomechanical testing. The ameliorated bone healing concurred with the augmented angiogenesis. These data demonstrated, that BMSCs engineered to express BMP2 and VEGF accelerate the repair of large femoral bone defects and improve the quality of the regenerated bone, which paves an avenue to utilizing baculovirus as a vector for BMSCs modification and regenerative medicine.

原文英語
頁(從 - 到)3222-3230
頁數9
期刊Biomaterials
31
發行號12
DOIs
出版狀態已出版 - 04 2010

指紋

深入研究「The healing of critical-sized femoral segmental bone defects in rabbits using baculovirus-engineered mesenchymal stem cells」主題。共同形成了獨特的指紋。

引用此