Thioredoxin-interacting protein: An oxidative stress-related gene is upregulated by glucose in human prostate carcinoma cells

See Tong Pang, Wen Chi Hsieh, Cheng Keng Chuang, Chun Hsiang Chao, Wen Hui Weng, Horng Heng Juang*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

24 引文 斯高帕斯(Scopus)

摘要

Thioredoxin-interacting protein (TXNIP), also known as vitamin-D3 upregulated protein 1, interacts with reduced thioredoxin. This protein modulates the cellular redox state and plays a role in stress-induced cellular apoptosis. This study examined TXNIP gene expression in prostate cancer cells. In vitro studies by immunoblot assay have shown that elevated glucose levels (1-15 mM) upregulate TXNIP gene expression two- to fourfold in human prostate carcinoma cells (LNCaP) and hepatocellular carcinoma cells (HepG2). Transient gene expression assays reveal that the promoter activity of the TXNIP gene is upregulated by glucose, 3-O-methylglucose, and maltose, but not by mannitol. These results suggest that glucose and 3-O-methylglucose induce TXNIP expression through both glucose metabolism-dependent and -independent pathways. Cotransfection of a plasmid expression carbohydrate response element-binding protein (ChREBP) with a TXNIP reporter vector into LNCaP cells dramatically enhances reporter activity in a low glucose (1 mM) condition. The effects of glucose are apparently mediated in a region located -341 to -324 bp upstream of the translational starting point of the TXNIP gene as indicated by 5′-deletion and site-directed mutagenesis reporter assays. Mutation of the putative carbohydrate response element (ChoRE) from CACGAGGGCAGCACGAG to TTTGAGGGCAGCACGAG abolishes glucose upregulation of TXNIP promoter activity. The present study demonstrates that TXNIP is transcription induced in both LNCaP and HepG2 cells in an increased glucose metabolism-dependent or -independent response, and a putative glucose regulatory system including ChREBP and ChoRE is needed for glucose-induced TXNIP gene in human prostate carcinoma cells.

原文英語
頁(從 - 到)205-214
頁數10
期刊Journal of Molecular Endocrinology
42
發行號3
DOIs
出版狀態已出版 - 2009

指紋

深入研究「Thioredoxin-interacting protein: An oxidative stress-related gene is upregulated by glucose in human prostate carcinoma cells」主題。共同形成了獨特的指紋。

引用此