Translocation of nucleolar phosphoprotein B23 ( 37kDa pI 5.1) induced by selective inhibitors of ribosome synthesis

Benjamin Yat Ming Yung*, Harris Busch, Pui Kwong Chan

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

153 引文 斯高帕斯(Scopus)

摘要

To elucidate the possible role of nucleolar phosphoprotein B23 in ribosome synthesis, drugs which inhibit the processing of ribosomal RNA were employed. After treatment with actinomycin D, toyocamycin or high doses of α-amanitin, a uniform nucleoplasmic fluorescence was observed. Low doses of α-amanitin and the protein synthesis inhibitor puromycin and cycloheximide had no effect on protein B23 translocation. By ELISA immunoassay, there was a 60% decrease in the amount of protein B23 in the nucleoli of the actinomycin D-treated cells as compared with the control nucleoli. Conversely, the amount of protein B23 in the nucleoplasm (excluding nucleoli) was 3-fold higher in the actinomycin D-treated cells. Preribosomal ribunucleoprotein particles (pre-rRNPs) were extracted from isolated nucleoli of Novikoff hepatoma ascites cells and fractionated on sucrose density gradients. Protein B23 was found co-localized with the pre-rRNPs as determined by ELISA assays which agrees with previous studies. The proteins in these 80 S and 55 S pre-ribosomal ribonucleoprotein particles were fractionated by 10% gel electrophoresis. Immunoblots showed protein B23 was present in both pre-rRNPs.

原文英語
頁(從 - 到)167-173
頁數7
期刊BBA - Gene Structure and Expression
826
發行號4
DOIs
出版狀態已出版 - 18 12 1985
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