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Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction

  • Yung Chin Chung
  • , Jheng Syong Wu
  • , Chien Chou*
  • *此作品的通信作者
  • Chang Gung University

研究成果: 圖書/報告稿件的類型會議稿件同行評審

摘要

A two-frequency heterodyne confocal laser scanning fluorescence microscope (TF-CLSFM) is setup where a harmonic intensity modulated fluorescence signal is detected coherently. Due to the ability of reduction on refractive index mismatched spherical aberration in TF-CLSFM, it clearly shows that the performance of TF-CLSFM on axial response is better than conventional confocal laser scanning fluorescence microscope (CLSFM) according to the experimental results.

原文英語
主出版物標題Three-Dimensional and Multidimensional Microscopy
主出版物子標題Image Acquisition and Processing XXI
發行者SPIE
ISBN(列印)9780819498625
DOIs
出版狀態已出版 - 2014
事件Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI - San Francisco, CA, 美國
持續時間: 03 02 201406 02 2014

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
8949
ISSN(列印)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI
國家/地區美國
城市San Francisco, CA
期間03/02/1406/02/14

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