Using control microfluidic system to enhance the sperm motility sorting efficiency

Hsin Yao Tseng, Da Jeng Yao, Chang Hung Tien, Chin Jung Li, Hong Yuan Huang

研究成果: 圖書/報告稿件的類型會議稿件同行評審

摘要

A microfluidic device was designed with two parallel laminar-stream-based surface-modified microchannels to separate motile sperm. This modified sperm sorter provided a new selection that had advances in resolving traditional sorting problems. Microscopic examination showed that sperms with different motility and the results of fluorescence images indicated that the laminar streams are the same as the CFDRC simulation result which revealed distribution of flow field. For the purpose of confirming the real sorting situation, the proportions of living and dead sperm after sorting were readily identified and quantified through use of flow cytometric analysis. Moreover, we present a control microfluidic system to change the flow rate. The controlmicrofluidic system is consists of syringe pump and connective tube module. Decreasing the velocity of flow field provide more opportunities of sorting sperm. The result showed that the sperm collected via our device have higher activity on average. As a result, we can enhance the sorting efficiency in the quality and quantity of sperm. With this approach, the characteristics of laminar flow and a control microfluidic system were utilized to demonstrate the microfluidic system with high efficiency for sperm motility sorting.

原文英語
主出版物標題NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
頁面47-50
頁數4
DOIs
出版狀態已出版 - 2012
對外發佈
事件6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, 泰國
持續時間: 04 11 201207 11 2012

出版系列

名字IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN(列印)2159-6964
ISSN(電子)2159-6972

Conference

Conference6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
國家/地區泰國
城市Bangkok
期間04/11/1207/11/12

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